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115-035-003Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) 山羊抗小鼠现货

115-035-003Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) 山羊抗小鼠现货
  • 115-035-003Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) 山羊抗小鼠现货
商品介绍

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Target: MouseHost: GoatAntibody Format: Whole IgG Specificity: IgG (H+L)Conjugate: Horseradish PeroxidaseProduct Category: Whole IgG Affinity-Purified AntibodiesClonality: Polyclonal RRID: AB_10015289
品牌介绍

Time Bioscience是一家主要生产Wnt蛋白和Wnt报告基因细胞系的厂家。Wnt蛋白主要的客户群体有药物研发、毒理研究、基础研究以及Wnt相关的药物研发。客户的研究方向有干细胞生物、类器官培养以及干细胞治疗。

Wnt蛋白有19种,比较常见的是Wnt3a和wnt5a。和其他品牌人的wnt3a相比,Time Bioscience是在人的细胞系中表达的,没有改变蛋白的序列,虽然蛋白的表达产量下降,但是其蛋白的活性是其他品牌的10~20倍。


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